Objectives: The linearity of neural information is widely accepted in the study of neuroscience. However, the examinations on the transmitted linearity of neural information are elusive. Here, we explored the traditional assumption by investigating the linearity of transmitted information by multiple stimulations on the vestibular system, specifically the vestibular nucleus. Methods:Five healthy guinea pigs were used for this study. The fixed animals head in a stereotaxic apparatus following anesthesia was placed on the top of a horizontally rotatory table. A combined stimulation with GVS and rotation was applied for the neuronal responses to the multiple stimulations. The neuronal responses were tested in four separated stages; a resting, a pure head rotation, a pure GVS and the combined stimulus stage. We curve- fitted a sine wave on the neuronal firing rate (FR) during rotation and a linear regression during the resting and GVS. The linearity of neural information was based on the calculated FR difference from the stages. Result:The neuronal responses were analyzed based on the curve-fitting and average FR during the stages. To test the linearity of neuronal information, we compared the average FRs during the resting and the pure GVS stages. At the same time, the difference between the sine wave baselines during the pure rotation and the multiple stimulation. The peak-to-peak amplitude of the curve-fitted sine wave decreased during the combined stimulus stage, compared with that during the pure rotation. The difference between each pair indicated that the linearity of neuronal information was maintained in the VN under the multiple stimulation. Conclusion:We could identify the linearity of neuronal information in the VN through the comparisons of sine wave baselines and average FR. The difference between two baselines during the pure rotation and the multiple stimulation indicated the neural information is transmitted with reduced amplitudes, but the linearity of transmitted information was maintained.