목적: Stem cell therapy may provide a means to reduce radiation-induced hyposalivation and improve the quality of life of patients. However, the leakage of stem cell from the gland is one of problem. We attempted to determine whether hydrogel mixed Mesenchymal Stem Cell (MSC) can ameliorate radiotherapy (RT)-induced salivary gland damage more effectively. 방법:Male C57BL/6 mice were divided into five groups, group A (normal; n = 5), group B (RT only; n = 5), Group C (RT + MSC; n=5), Group D (RT + Hydrogel; n=5), and group E (RT + MSC + hydrogel; n = 5). External irradiation at a dose of 15 Gy was delivered to the neck fields. At 6 weeks post-transplantation, we measured salivary gland weights, salivary flow rates (SFR), and performed histological and immunofluorescent examinations. In addition, changes in salivary uptake and excretion were observed by single-photon emission computed tomography (SPECT). 결과:SFR was significantly increased in group E mice compared with Group B, C, D mice at 6 weeks after transplantation. Administration of hydrogel mixed MSC preserved the microscopic morphologies, with more functional acini. Immunofluorescent staining revealed less apoptotic cells and increased microvessel density in hydrogel mixed MSC group. Tc-pertechnetate excretion rate was significantly better in group E in SPECT analysis. 결론:Hydrogel mixed MSC injection can prevent salivary damage following irradiation more efficiently and can be used as a source of cell-based therapy for restoration of irradiation-induced salivary hypofunction.