목적: The mouse is the most popular animal model in olfactory research covering anatomy, physiology, genetics, neural connection, and functional phenotype. Among them, behavior test with odorant is of essential to determine olfactory phenotype. So far, to our knowledge, mouse olfactory behavior test has not been standardized and its result could be affected by inter-observer variations. We tried to develop new mouse olfactory behavior test that can be assessed by automatic video tracking system. 방법:The Smart 3.0 video-tracking software (Harvard apparatus©) and video camera system was used to track automatically mouse behavior in the standard breeding cage. Olfactory sensitivity was assessed in C57BL/6N, C57BL/6J, Balb/c, and 129S strains of male/female mouse.Three times of habituations without odorant were performed in the same cage just before experiment. We tested with basically two odorants (peanut better for preference test, 2MB for avoidance test) and water (for control). The mouse behavior was recorded for 3 minutes and analyzed by software. For preference test, investigation time (how quickly find out the odorant) was measured. For avoidance test, time spent in odorant zone (how long avoid the odorant) was measured. To confirm our experimental setting, anosmia mouse model prepared with intranasal administration of ZnSO4 was also evaluated. 결과:All strains of mouse showed reproducible behavior pattern of preference or avoidance for odorants. Water attracted mice vaguely and it was in-between preference and avoidance. Anosmia mouse model, as our expectation, failed to show olfactory ability of preference or avoidance. Automatic video tracking system successfully tracked mouse behavior and calculated accumulative time until targeted point with good reproducibility. 결론:Our olfactory behavior test seems to be simple and accurate. We suggest that our behavior test can be utilized as a possible standard method to search for features of olfactory phenotype in mouse model.