목적: Olfactory transduction begins at the level of the olfactory receptor neurons located on the nasal turbinate. It would be very useful to trace neuronal connections from olfactory epithelium to specific functional areas of the brain. Mn2+ is known to enter excitable cells on calcium channels that open due to specific stimulation. Mn2+ is also transported long neurons by microtubule-dependent axonal transport. We tried to establish functional MRI using MnCl2 to investigate olfactory bulb and its tract in mouse model. 방법:MnCl2 was used as a source of Mn2+ and it was administered Intranasally and MRI was taken at different time(0~24h after MnCl2 instillation). Initially mouse was anesthesized with inhalation of isoflurane before moving into MRI cradle. In the cradle, mouse was positioned in prone and body temperature was maintained at 37.5°C. Isoflurane was continuously provided through gas tube to maintain anesthesia. 9.4T magnet(Bruker), especially designed for mouse or rat, was utilized. C57BL6/J mice were used and anosmia model was also prepared with ZnSO4 before experiment to compare with normal mouse. 3D T1-weighted MRI sequences were obtained and the images were analyzed by imaging software. 결과:Manganese-enhancement was observed at the interface between the olfactory nerve layer and olfactory turbinate. As time passes, highlighting of the outer layers of olfactory bulb where olfactory glomeruli and mitral cell are located was noted. In addition, the enhanced contrast continues caudally into the primary olfactory cortex. In contrast, anosmia mouse model failed to show enhancement at the interface between olfactory bulb and turbinate, glomeruli and mitral cell layer of olfactory bulb, and connection to primary olfactory cortex. 결론:The manganese-enhanced functional MRI is a useful method to map neuronal function and connections. For further study, comparing the enhancement patterns according to different odorants, mouse strain or gene manipulation is currently underway.