목적: Primary nasal epithelial cells (NEC) are used for diagnostic purposes in clinical routine and have been shown to be good surrogate models in studies of nasal inflammation, allergic rhinitis and immune responses against nasal pathogens. However, for cell culture, it is necessary to obtain human tissue invasively for every experiment, and it takes a long time to establish a full differentiation, so there are still many limitations in using it for repeated in vitro experiments. We describe a NEC organoid model derived from human nasal samples that is well- differentiated and recapitulates the airway epithelium with multiple outcome measurements depending on the application of respiratory virus. 방법:Eight organoids derived from five human nasal mucosa (NB35-NB39) and three nasal brushing (NB06-111N-113N) were first cultured and the culture has been completed until passage 3. 결과:The histologic results showed that full differentiated nasal epithelium was obtained at least 14 days after confluence in ALI culture systems. Both ciliated and secretory cells were observed through H&E and PAS staining and secreted mucus was also found in organoid cultures. Transmission electron micrographs also showed well differentiated cilia and secretory mucus and the findings of scanning electron microscope revealed that well differentiated cilia, mucin granules and tight cellular junctions. The mRNA levels of specific cellular markers for basal progenitor cells, nasal epithelial cells, secretory cells, and ciliated cells were measured and our NEC organoid models showed a significant expression of TP63, KRT5, MUC5AC, and FOXJ1 genes until passage 3. 결론:This highly differentiated nasal epithelial organoid model could serve as a surrogate biomarker to assess the characteristics of nasal epithelium, recapitulating the phenotypic and genotypic diversity against external stimulations such as respiratory pathogens, allergens and cytokines.