목적: We analyzed the effects of photobiomodulation (PBM) of various wavelengths on regeneration of the facial nerve using in vitro and in vivo experimental models. 방법:Geniculate ganglion neurons were harvested from P4 rats and were cultured. Various wevelengthes of photobiomodulation was applied after 24 hours of H2O2 treatment. For vivo study, SD rat was used and facial nerve palsy was induced by clamping and cutting of facial nerve trunk. Two wavelength include 633nm or 804 nm of phoiomtobiomodulation was irradiated after facial nerve damage. At the end of experiment, functional and histological analysis were performed. 결과:Wavelengths of 633 and 780 nm but not 804 nm inhibited cell death by oxidative stress. We assessed the effects of PBM on functional and morphologic recovery in rats divided into control, facial nerve damage (FND) and FND irradiated with a 633 nm or 804 nm lasers. Injured rats treated with 633-nm light had better facial palsy scores, larger axon diameter and higher expression of Schwann cells compared with the FND group. No positive results were observed in rats irradiated at 804-nm light. 결론:These findings suggests that 633-nm PBM promotes accelerated nerve regeneration and improved functional recovery in an injured facial nerve.